Drug encyclopedia

This prescription is copied directly from The Turn-On Book, BarNel Enterprises. Working with mushrooms, it is very important to use a method of reception of “pure growth”, that is without polluting mushrooms an undesirable mould. This method of pure growth can be comprehended easily, having read any practical guidance on work in bacteriological laboratory. Any who took place a biology course, will easily show a method of transplantation of a mould and preparation of “a vaccinating loop” which allows to tolerate fungi from one pan or a flask to another, without stuff infestation.

Cautious transplantation of fungi psilotsiby – most an important point as a psilocybe it is easily blasted and acquires other mould which is present at environment. A stuff on which grows a psilocybe, routinely name “medium”. Preparation of medium differs for different kinds a little, but the approach is identical to all. Ingredients under the list are weighed (the special accuracy it is not required), solve in the necessary quantity of water and are seated in the container for degermination. It is possible to use fruit jars in volume about a pint or a quart, having covered with their serious grid from an aluminium foil.

As the medium is going to cultivate on purpose pure growth, presence of other microorganisms which can sprout too, should be excluded. Therefore the medium before utilisation needs to be sterilised to kill bacteria and spores of a mould which can appear in medium or on pot glass. Degermination needs to be made in an autoclave within 15-20 minutes at 250С. To breed demanded pressure in an autoclave follows very sluggishly that the medium was not splashed out.

Fruit jar in volume on a quart, should seat no more than two beakers of the used medium, in jar in volume on a pint it is necessary to put 3/4 beakers of medium. The medium with available in it a сахаром (a glucose, sucrose, a maltose, etc.) should heat up about 20 minutes at 250С for caramelization. This caramelization can appear pernicious for a mould – it can not grow or will cultivate it is not enough. It is possible not to receive at all psilocybes.

After preparation and degermination, well to abandon medium for three days, without discovering it to be assured that the medium is really sterile. If it is not observed mould growth, and there are no Membranulas of bacteria on the medium (routinely visible or badly smelling), means degermination it has gone right. Otherwise all should be thrown out. Never and any medium it is not possible to sterilise repeatedly for cultivation psilocybes.

To receive medium where it is possible to cultivate to a psilocybe a long time, it is possible to prepare some tubes with agar-agar as curing agent. Tubes in length of 6 inches and about 1/2 inches in diameter, with the screw and rubber strips (they can be found in any laboratory, it is tubes) are most convenient.

To fill tubes on 1/3 medium with agar-agar (after agar-agar – fusion formulas see), to sterilise and cool to ambient temperature for agar-agar hardening. To treat fungi water on procedure, for pure growth reception. To sustain tubes at ambient temperature within several days – even week – until on a surface the mould will seem. Covers to take out and seat cultures on storage in a refrigeration cabinet. It will be “a depot of cultures” and a source of working out of various kinds of medium. Application of a depot of cultures provides constant presence of suitable, not infested stuffs. A psilocybe it is stored in a refrigeration cabinet about one year, without demanding new medium.

To tolerate to more capacious containers a small amount of thin white strands (so-called “mycelium” or a mycelium), applying a pure growth method. To abandon culture at ambient temperature 70-75F. It to make very simply if there is a cellar or if to deliver a thermostat in a usual refrigeration cabinet that it was convenient to maintain the necessary temperature. Mushrooms sprout psilocybes and at more heat, but the psilocybine content in them is depressed, and even at all is absent.

It is not obligatory to wait, while mushrooms will be issued (named a fungal body) to take a psilocybine. In myceliums contains it as much, how many and in a fungal body. When mushrooms have grown till 10-12 days, they can be collected. (This time – the changeable factor for reception of the maximum yield of a psilocybine. The yield will be necessarily affected by all path and errors of individual conditions of growth).

It is necessary to do carefully records about used mediums, temperature and collecting time. Records can help for enriching of a practical yield psilocybes. On a science to collect mushrooms follows for the fourth day after the mould will eat all sugar. Reaping crop, it is necessary to delete medium: a fluid medium to delete filtering through a column, collecting mycelium lumps, and a solid medium – simply collecting mycelium chunks.

The collected mycelium cautiously to dry up at small warming (not above 200F in the furnace with slightly slightly opened door) and after that to erase in a powder dry matter. It is possible to make powder extraction, having presoaked it in a methanol, having filtered off and having vaporized fluid at small warming. It is necessary to do it in well aired room, until then all methanol has exhaled.

The psilocybine will particulate pass and on medium, but in very small amount, attempts to take have no its success.

The procedure set forth above seems simple, but after several attempts there is an interference with certain difficulties. Psilocybine effecting depends on many factors the part from which is unknown till now. There is no other path, except assays and errors in perfection of medium and methods.